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class="NyNewDetail_Content"> <h3 style="text-align:center"><span style="color:#FF0000"><strong><span style="font-size:22px">无菌室操作流程参考上海</span></strong></span></h3><p> </p><p><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">无菌试验室是细菌试验室内用于无菌操作的小室，其内部装饰、消毒条件要求更严格。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">1、无菌室应封闭，人员出入应有两道门，其间应隔有缓冲区。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">2、用前应以紫外线消毒30min，定期用乳酸或甲醛熏蒸，消毒。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">3、在无菌室中一般于分装无菌的培养基及传染性强的细菌的接种，不进行有菌标本的分离及其他操作。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">4、无菌试验室内应操作人员进入，而且进入无菌室应着隔离衣和鞋，操作时戴口罩，随时保证室内的无菌状态。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">5、条件有限的试验室，可用净工作台无菌试验室进行相应的操作。净工作台应选择垂直气流通风方式。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">6、无菌室应配备空调设备，保证不因室温而影响工作医学教育网整理。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">操作程序：</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">1、试验前将被检样品及试验用品放入传递窗，关闭外门，打开紫外灯，照射30分钟。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">2、打开过滤风机、紫外灯和电源开关，紫外灯照射30分钟。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">3、工作人员进入工作室前洗手，在缓冲间内穿戴无菌衣、帽、口罩、更换拖鞋后，通过风淋室，全身各面进行风淋，进入工作室。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">4、用消毒剂消毒手和腕部，将所需物品经传递窗传进。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">5、工作期间不得进出工作室。试验结束后，即时清洁台面和地面，室内留用物品摆放整齐，其他物品经传递窗传出。工作人员关好内门，进入缓冲间，更换拖鞋和无菌衣，离开工作室，打开紫外灯照射30分钟。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">6、打开传递窗，取出接种好的培养物送去培养，剩余样品根据试验室有关规定妥善处理。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">7、关闭风机和紫外灯电源。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">空调器使用说明：</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">1、洁净试验室内空气需加热或制冷，按下列方法进行。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">2、按空调?？仄?ldquo;ON/OFF”键，根据需要选择加热或制冷模式。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">3、按温度设定键▲或▼可在16℃～30℃范围之间每次增高和降低1℃设置温度。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">4、?？仄髅姘迳掀渌醇话闱榭鱿虏槐厥褂谩?/span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">维护管理：</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">1、与检测工作无关的人员未经检验室负责人批准一律不得入内，检测人员不得在洁净工作室内从事与检验工作无关的其他活动。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">2、每次试验完毕，认真检查电灯、空调器等开关，使其处于关闭状态，严防事故隐患。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; font-size:12px">3、保持洁净工作室内整洁卫生，室内物品摆放整齐，除每次试验完毕消毒工作台面外，每个月用消毒液洗抹室内桌椅、地面和墙壁一次。</span><br /><span style="color:rgb(0, 0, 0); font-family:宋体; 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